Cancer Stem Cells Recapitulates the Heterogeneity of Glioblastomas
نویسندگان
چکیده
Federica Colleoni1, Marzia Belicchi1, Paola Razini1, Mirella Meregalli1, Rossella Galli2, Stefania Mazzoleni2, Silvia Brunelli2, Marina Di Segni3, Domenico Coviello4, Marco Baccarin3, Bianca Pollo5, Nereo Bresolin1, Lorenzo Bello6, Luciano Conti7 and Yvan Torrente1* 1Stem Cell Laboratory, Department of Pathophisiology and Transplantation, Università degli Studi di Milano, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, via F. Sforza 35, 20122 Milan, Italy 2Stem Cell Research Institute (SCRI) DIBIT-H. S. Raffaele via Olgettina 60, 20132 Milan, Italy 3Laboratory of Medical Genetics, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, via F. Sforza 35, 20122 Milan, Italy 4Laboratory of Human Genetics and Department of Experimental Medicine, University of Genoa, Genoa, Italy 5Divisione di Neurologia–Neuropatologia V Istituto Neurologico C. Besta, via Celoria 11, 20133 Milan, Italy 6Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, Milan, and Humanitas Research Hospital, Rozzano, MI, Italy 7Centre for Integrative Biology, Università degli Studi di Trento, Trento, Italy
منابع مشابه
Heterogeneity of the Level of Activity of Lgr5+ Intestinal Stem Cells
Intestinal stem cells (ISCs) are a group of rare cells located in the intestinal crypts which are responsible for the maintenance of the intestinal homeostasis and intestinal regeneration following injury or inflammation. Lineage tracing experiments in mice have proven that ISCs can repopulate the entire intestinal crypt. It is noteworthy that in such experiments, only a subset of intestinal cr...
متن کاملA Three-Dimensional Organoid Culture System Derived from Human Glioblastomas Recapitulates the Hypoxic Gradients and Cancer Stem Cell Heterogeneity of Tumors Found In Vivo.
Many cancers feature cellular hierarchies that are driven by tumor-initiating cancer stem cells (CSC) and rely on complex interactions with the tumor microenvironment. Standard cell culture conditions fail to recapitulate the original tumor architecture or microenvironmental gradients and are not designed to retain the cellular heterogeneity of parental tumors. Here, we describe a three-dimensi...
متن کاملCurrent Strategies for Identification of Glioma Stem Cells: Adequate or Unsatisfactory?
Cancer stem cells (CSCs) were isolated in multiple tumor types, including human glioblastomas, and although the presence of surface markers selectively expressed on CSCs can be used to isolate them, no marker/pattern of markers are sufficiently robust to definitively identify stem cells in tumors. Several markers were evaluated for their prognostic value with promising early results, however no...
متن کاملCD133 negative cancer stem cells in glioblastoma.
Glioblastomas (GBM) are paradigmatic for the investigation of cancer stem cells (CSC) in solid tumors. Recently, the discovery of CD133- CSC in addition to CD133+ CSC has substantially added to our understanding of the complexity of GBM CSC. This review gives an overview on our current knowledge on CD133- cells in GBM and describes five different hypothesizes on the nature of CD133- cells in GB...
متن کاملW4: Cancer Stem Cells as a New Target Point for Treatment of Glioblastoma
Glioblastoma is a destructive form of brain tumor that kills most patients within two years of diagnosis.Treatments for glioblastoma include the usual options of surgery, radiation therapy and chemotherapy, but there is no effective treatment. The tumors are capable of spreading tendrils out into the brain and it can grow back in a matter of months after being removed. The cancer stem cell...
متن کاملHuman Cancer Modeling: Recapitulating Tumor Heterogeneity Towards Personalized Medicine
Despite diagnostic, preventive and therapeutic advances, growing incidence of cancer and high rate of mortality among patients affected by specific cancer types indicate current clinical measures are not ideally useful in eradicating cancer. Chemoresistance and subsequent disease relapse are believed to be mainly driven by the cell-molecular heterogeneity of human tumors that necessitates perso...
متن کامل